In vitro propagation of jojoba.

In vitro propagation of some promising genotypes of jojoba (Simmondsia chinensis)

Nodal segments (1.5 3.0 cm long) of six promising jojoba genotypes were cultured in vitro on solidified MS medium supplemented with BA in combination with different concentrations of NAA, IAA or IBA for shoot formation. The plant growth regulators combination of 5.55 μM BA + 7.1 μM IAA proved the best for shoot initiation and subsequent growth. Some explants of the jojoba genotypes, PKJ-3 and P...

Molecular-based marker for sex differentiation of jojoba in vivo and in vitro Iraqi cultivars using RAPD- PCR technique

The objective of this study was to distinguish between males and females of jojoba (Simmondisia chinensis) using Random Amplified Polymorphic DNA (RAPD) markers in order to detect any DNA variation. A callus was produced from both male and female jojoba leaves, using MS medium with BA 0.5 and 2 to 4, D 2.5 mg/l respectively. Genomic DNA of jojoba plant was extracted using CTAB method. The conce...

Effect of Antioxidants, Amino Acids and Plant Growth Regulators on in vitro Propagation of Rosa centifolia

Background: Rosa centifoliais commercially propagated by asexual means but in vitro propagation ensure the production of disease free and healthy plants and browning of explants creates hurdle in their multiplication. Objectives: The aim was to reduce oxidative browning of shoots of R. centifolia in MS medium during in vitro propagation. Materials and Methods: Axillary buds of R. centifolia we...

Comparisons of in vitro propagation of tomato cultivars

Rapid in vitro propagation of Stevia rebaudiana Bertoni and evaluation of its stevioside content

An efficient protocol was established for rapid in vitro propagation of Stevia rebaudiana Bertoni, using explants from mature plants. The effect of 1/2MS, MS and B5 culture media, also 27 different combinations of auxins and cytokinins was studied on in vitro growth and development of Stevia rebaudiana Bertoni. The best culture medium for shoot and root formation was 1/2MS, producing 1.30 shoot...